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Study of anticlastogenic effect of spirulina against arsenic using zebra fish by micronucleus method


Sayantani Chanda1 *, T. V. Ramachandra2, Puspal Dey3 , Madhumita Joarder Mukhopadhay4

1,2, Energy & Wetlands Research Group, Centre for Ecological Sciences [CES],
Indian Institute of Science, Bangalore, Karnataka, 560 012, India,
Web URL: http://ces.iisc.ernet.in/energy; http://ces.iisc.ernet.in/foss,
3,4, Maulana Abul Kalam Azad University of Technology, Kolkata, (W.B.) - India
*Corresponding author:E.mail: isayantani.chanda@gmail.com

Results and Discussion

Micronucleus Frequency

From the experiment we observed, when treated with only sodium arsenite, total number of normal cells 180, micronucleus 59 and abnormalities 32.77%. Again when treated with only spirulina the total number of normal cells 185, micronucleus 16 and abnormalities 8.64%. Result of Control gives total number of normal cells 170, micronucleus 5 and abnormalities 2.94%.Micronucleus frequency always increases with time in fish in relation to genotoxicity, which was greater when treated with as than in fish treated with spirulina. When treated with both (sodium arsenate + Spiraling) the cells turn over sub lethal condition decreases micronucleus frequency, total number of normal cells 171, micronucleus 27 and abnormalities 15.78%. The micronucleus observations are showed on Figure 1.The final results showed in the Table 1 and Figure 2(a,b,c).

Fig 1:Micronucleus

Sl.No Chemical Name Total no. of normal cells Micronucleus cells % of abnormality
1 Only spirulina 185 16 8.64%
2 Only Sodium Arsenite 180 59 32.77%
3 Sodium Arsenite+S pirulina 171 27 15.78%
4 Control 170 5 2.94

Table 1: Results of the study

Fig. 2(a): Bar diagram represent comparisons between the expose treatment and controls shows the highest number of total normal cells

Fig. 2(b): Bar diagram represent comparisons between the expose treatment and controls shows the highest number of micronucleus

Fig. 2(c): Bar diagram represent comparisons between the expose treatment and controls shows the highest percentage of abnormalities